1,617 research outputs found

    DNA amplified fingerprinting, a useful tool for determination of genetic origin and diversity analysis in Citrus

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    We used three short repetitive nucleotide sequences [(GTG)5, (TAC)5, and (GACA)4] either as radiolabeled probes for hybridization with restricted Citrus DNA or as single primers in polymerase chain reaction amplification experiments with total genomic DNA. We tested the ability of the sequences to discriminate between seedlings of zygotic or nuclear origin in the progeny of a Volkamer lemon #Citrus volkameriana# Ten. & Pasq.) tree. The genetic variability within two species [#Citrus sinensis# (L.) Osbeck (sweet oranges) and #Citrus reticulata# Blanco and relatives (mandarins)] was evaluated. DNA amplified figerprinting with single primers was the more successful technique for discriminating between nucellular and zygotic seedlings. Although we were not able to distinguish among 10 cultivars of #C. sinensis#, all 10 #C. reticulata# cultivars tested were distinguishable. However, it still is difficult to identify the putative parents of a hybrid plant when the two parental genomes are closely related. (Résumé d'auteur

    The changing UV and X-ray properties of the Of?p star CPD -28 2561

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    The Of?p star CPD -28 2561 was monitored at high energies with XMM-Newton and HST. In X-rays, this magnetic oblique rotator displays bright and hard emission that varies by ~55% with rotational phase. These changes occur in phase with optical variations, as expected for magnetically confined winds; there are two maxima and two minima in X-rays during the 73d rotational period of CPD -28 2561. However, contrary to previously studied cases, no significant hardness variation is detected between minima and maxima, with the exception of the second minimum which is slightly distinct from the first one. In the UV domain, broad-band fluxes remain stable while line profiles display large variations. Stronger absorptions at low velocities are observed when the magnetic equator is seen edge-on, which can be reproduced by a detailed 3D model. However, a difference in absorption at high velocities in the CIV and NV lines is also detected for the two phases where the confined wind is seen nearly pole-on. This suggests the presence of strong asymmetries about the magnetic equator, mostly in the free-flowing wind (rather than in the confined dynamical magnetosphere).Comment: 14 pages, 11 figures, accepted for publication by MNRA

    Towards Transparency of IoT Message Brokers

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    In this paper we propose an ontological model for documenting provenance of MQTT message brokers to enhance the transparency of interactions between IoT agents
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